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| Registros recuperados: 233 | |
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| Acharya,Laxmikanta; Mukherjee,Arup Kumar; Panda,Pratap Chandra. |
| Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. senso lato into three distinct genera namely Chamaecrista Moench., Senna P. Mill. and Cassia L. sensostricto Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci) respectively. Most of the loci are found to be polymorphic, showing high degrees of genetic diversity among the different taxa studied. The dendrogram constructed on the basis of the RAPD, ISSR and AFLP data using SHAN clustering, divided Cassia L.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP; Cassia; ISSR; Molecular phylogeny; RAPD. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000100026 |
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| Almeida,Álvaro M. R.; Abdelnoor,Ricardo V.; Arias,Carlos A. Arrabal; Carvalho,Valdemar P.; Jacoud Filho,David S.; Marin,Silvana R. R.; Benato,Luís C.; Pinto,Mauro C.; Carvalho,Cláudio G. P.. |
| Macrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; ITS; PCR-RFLP; Soybean charcoal rot. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000300009 |
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| Almeida,Álvaro M. R.; Piuga,Fernanda F.; Marin,Silvana R. R.; Binneck,Eliseu; Sartori,Fábio; Costamilan,Leila M.; Teixeira,Maria R. O.; Lopes,Marcelo. |
| Cercospora kikuchii, involved with the defoliation of soybean (Glycine max) plants, is normally associated with Septoria glycines in late season. Seventy-two isolates from different regions in Brazil, obtained mainly from purple stained seeds, showed phenotypic variation. Cercosporin content and rate of colony growth was higly variable among isolates. A strong correlation between cercosporin content and virulence was identified. Genetic variation among and within populations was evaluated based on 86 RAPD loci. The RAPD analysis clustered all isolates into seven groups. No relationship was observed between RAPD groups and geographic origin or cercosporin content. The sequence of the internal spacer regions (ITS1-5.8S-ITS2) from 13 isolates chosen according... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotypic diversity; PCR-RFLP; RAPD; ITS sequence; Virulence. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000600005 |
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| Almeida,Luiz Carlos C.; Coêlho,Rildo S.B.. |
| A antracnose é a doença pós-colheita mais importante do maracujá amarelo, cujo agente etiológico, no Brasil, foi identificado como Colletotrichum gloeosporioides. Visando caracterizar o patógeno, foram obtidos 33 isolados de três regiões produtoras do estado de Pernambuco. Critérios morfológicos como cor de colônia, forma e dimensão de conídios, a produção de peritécio e o uso de primers específicos para C. acutatum, C. gloeosporioides e "Colletotrichum de Passiflora" permitiram identificar Glomerella cingulata patótipo 1, G. cingulata patótipo 2, Colletotrichum sp. de Passiflora e Colletotrichum sp. de maracujá amarelo. Inoculações em maracujá amarelo possibilitaram separar os isolados em dois grupos, um de agressividade alta (GA-1) e outro de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passiflora edulis f. flavicarpa; Antracnose; Pós-colheita; RAPD; PCR. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400006 |
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| Almeida,Margarete Teresa Gottardo; Rubio,Fernando Gôngora; Garcia,Doroti Oliveira; Pavarino-Bertelli,Érika Cristina; Rossit,Andrea Regina Baptista; Bando,Silvia Yano; Silbert,Suzane; Goloni-Bertollo,Eny Maria; Soares,Márcia Maria Costa Nunes; Martinez,Marina Baquerizo. |
| Stenotrophomonas maltophilia is a Gram-negative bacillus, which is becoming widely recognized as an important nosocomial pathogen. The main objective of this study was to evaluate the genetic relatedness, by random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of 86 clinical isolates of S. maltophilia (colonization 22, infection 64) obtained from 79 hospitalized patients, from different geographic regions of São Paulo State. The genotypic analysis performed by RAPD and PFGE was used in 24 isolates for genetic identity confirmation. The results were congruent between the two methods but it was not possible to link genetic profiles with the studied variables, clinical state and geographic area, probably due to the great... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stenotrophomonas maltophilia; RAPD; PFGE. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200017 |
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| Almeida,Mariana Castro da Costa; Chiari,Lucimara; Jank,Liana; Valle,Cacilda Borges do. |
| A utilização de marcadores moleculares pode servir para direcionar cruzamentos, confirmar novos híbridos e identificar genótipos para fins comerciais. Nesse contexto, o objetivo deste trabalho foi analisar a diversidade genética entre cultivares e híbridos de Brachiaria spp. e Panicum maximum usando marcadores moleculares do tipo RAPD (Polimorfismos de DNA amplificados ao acaso). Foram 22 genótipos analisados com 10 primers, os quais amplificaram 178 fragmentos polimórficos de DNA, que foram usados para estimar a similaridade genética por meio do coeficiente de Jaccard. Os valores de similaridade obtidos variaram de 0,066 a 0,841. A estrutura genética entre todos os genótipos estudados foi estimada pelo método UPGMA (Método de agrupamento com médias... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brachiaria spp.; Forrageiras; Marcadores moleculares; Panicum maximum; RAPD. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001100024 |
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| Areias,Rogeria Gregio de Biase Martins; Paiva,Diogo Mendes de; Souza,Sonia Regina; Fernandes,Manlio Silvestre. |
| Em experimentos em casa de vegetação e câmara de crescimento, estudou-se o possível aparecimento de réplicas em 20 variedades crioulas de arroz do Maranhão, mantidas no Banco Ativo de Germoplasma (BAG), do Centro Nacional de Pesquisa em Arroz e Feijão (CNPAF). As vinte variedades, divididas em seis grupos, com nomes similares e números de acessos diferentes no BAG foram avaliadas quanto à similaridade morfológica e molecular. As características morfológicas foram avaliadas e utilizadas para a construção de um dendrograma de similaridade. Após a colheita determinou-se o teor de proteína bruta dos grãos. No experimento em câmara de crescimento além das vinte variedades, utilizou-se a cultivar melhorada (IAC47). O DNA da parte aérea dessas plantas foi... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Similaridade genética; Características morfológicos; RAPD; Proteína bruta dos grãos. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052006000100004 |
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| Bandeira, Juliana Magalhães; UFPel; Bianchi, Valmor João; UFPel; Rubin, Sílvia; UFPel; Peters, José Antonio; UFPel; Braga, Eugenia Jacira Bolacel; UFPel. |
| The Plectranthus genus comprises several species generally referred to as boldo, which are highly used in popular medicine due to their anti-dyspeptic, analgesic and digestion-stimulating properties. The amount of natural active principles can vary greatly in the related genotypes, which may lead to the inappropriate use of these plants. This work aimed to analyze the interspecific diversity among four species of the Plectranthus genus (P. grandis, P. barbatus, P. neochilus and P. amboinicus) and the intraspecific diversity of P. barbatus collected from different places in southern Brazil, by means of the RAPD technique. A higher genetic similarity was observed between the P. neochilus and P. amboinicus species (80%), followed by P. grandis and P. barbatus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; Boldo; Genetic diversity; Medicinal plants Botânica; Genética Vegetal; Genética Molecular e de Microrganismos; Ciências Biológicas. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/880 |
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| Barroso,Paulo Augusto Vianna; Geraldi,Isaias Olívio; Vieira,Maria Lúcia Carneiro; Pulcinelli,Carlos Eduardo; Vencovsky,Roland; Dias,Carlos Tadeu dos Santos. |
| In order to verify whether genetic distance (GD) is associated with population mean (PM), genetic variance (GV) and the proportion of superior progenies generated by each cross in advanced generations of selfing (PS), the genetic distances between eight soybean lines (five adapted and three non-adapted) were estimated using 213 polymorphic RAPD markers. The genetic distances were partitioned according to Griffing's Model I Method 4 for diallel analysis, i.e., GDij = GD+ GGDi+ GGDj + SGDij. Phenotypic data were recorded for seed yield and plant height for 25 out of 28 populations of a diallel set derived from the eight soybean lines and evaluated from F2:8 to F2:11 generations. No significant correlation for seed yield was detected between GD and GV, while... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Soybean; Genetic distance; Molecular markers; RAPD; Prediction. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300020 |
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| Becerra Velásquez,Viviana; Paredes Cárcamo,Mario; Rojo Meriño,Carmen; France Iglesias,Andrés; Franco Durán,Jorge. |
| The genus Metarhizium consists of a diverse group of asexual entomopathogenic fungi, which have a wide geographical distribution. The Chilean National Agricultural Research Institute (Instituto de Investigaciones Agropecuarias - INIA, Quilamapu Chile) has collected about 350 isolates of Metarhizium anisopliae var. anisopliae from central and southern Chile. These isolates have been partially characterized using morphological traits such as conidia size and shape, colony color, growth pattern and the efficiency of the isolates in controlling specific pests. However, further characterization with molecular markers could detect differences in DNA which could help to better understand the genetic diversity and structure of Chilean populations of this fungus.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metarhizium; Genetic diversity; RAPD; SSR; ITS markers. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100017 |
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| Becerra V,Viviana; Paredes C,Mario; Rojo M,Carmen; France I,Andrés. |
| Los hongos entomopatógenos son una alternativa atractiva para el control biológico de insectos plagas. En Chile, el Instituto de Investigaciones Agropecuarias (INIA), Centro Regional de Investigación Quilamapu mantiene aproximadamente 400 aislamientos de Beauveria, colectados a través del país. Esta colección ha sido parcialmente clasificada en base a su morfología y a su eficacia como controlador biológico. Sin embargo, es necesario complementar estos estudios con una caracterización a nivel genético-molecular, para determinar el nivel de diversidad genética de Beauveria bassiana e identificar posibles patrones de bandas que permitan dicriminar entre aislamientos. En este estudio, se analizaron 36 aislamientos de B. bassiana provenientes de diversas... |
| Tipo: Journal article |
Palavras-chave: Hongos entomopatógenos; Diversidad genética; RAPD; Espaciadores internos transcritos (ITS). |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072007000200001 |
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| Registros recuperados: 233 | |
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